Compute full spectrum source space connectivity between labels#

The connectivity is computed between 4 labels across the spectrum between 7.5 Hz and 40 Hz.

Aud-rh, Vis-lh, Vis-rh
Reading inverse operator decomposition from /home/circleci/mne_data/MNE-sample-data/MEG/sample/sample_audvis-meg-oct-6-meg-inv.fif...
    Reading inverse operator info...
    [done]
    Reading inverse operator decomposition...
    [done]
    305 x 305 full covariance (kind = 1) found.
    Read a total of 4 projection items:
        PCA-v1 (1 x 102) active
        PCA-v2 (1 x 102) active
        PCA-v3 (1 x 102) active
        Average EEG reference (1 x 60) active
    Noise covariance matrix read.
    22494 x 22494 diagonal covariance (kind = 2) found.
    Source covariance matrix read.
    22494 x 22494 diagonal covariance (kind = 6) found.
    Orientation priors read.
    22494 x 22494 diagonal covariance (kind = 5) found.
    Depth priors read.
    Did not find the desired covariance matrix (kind = 3)
    Reading a source space...
    Computing patch statistics...
    Patch information added...
    Distance information added...
    [done]
    Reading a source space...
    Computing patch statistics...
    Patch information added...
    Distance information added...
    [done]
    2 source spaces read
    Read a total of 4 projection items:
        PCA-v1 (1 x 102) active
        PCA-v2 (1 x 102) active
        PCA-v3 (1 x 102) active
        Average EEG reference (1 x 60) active
    Source spaces transformed to the inverse solution coordinate frame
Opening raw data file /home/circleci/mne_data/MNE-sample-data/MEG/sample/sample_audvis_filt-0-40_raw.fif...
    Read a total of 4 projection items:
        PCA-v1 (1 x 102)  idle
        PCA-v2 (1 x 102)  idle
        PCA-v3 (1 x 102)  idle
        Average EEG reference (1 x 60)  idle
    Range : 6450 ... 48149 =     42.956 ...   320.665 secs
Ready.
Not setting metadata
72 matching events found
Setting baseline interval to [-0.19979521315838786, 0.0] s
Applying baseline correction (mode: mean)
Created an SSP operator (subspace dimension = 3)
3 projection items activated
Connectivity computation...
Preparing the inverse operator for use...
    Scaled noise and source covariance from nave = 1 to nave = 1
    Created the regularized inverter
    Created an SSP operator (subspace dimension = 3)
    Created the whitener using a noise covariance matrix with rank 302 (3 small eigenvalues omitted)
    Computing noise-normalization factors (dSPM)...
[done]
Picked 305 channels from the data
Computing inverse...
    Eigenleads need to be weighted ...
Processing epoch : 1 / 72 (at most)
Extracting time courses for 4 labels (mode: mean_flip)
    using t=0.000s..0.699s for estimation (106 points)
    frequencies: 8.5Hz..39.7Hz (23 points)
only using indices for lower-triangular matrix
    computing connectivity for 6 connections
    Using multitaper spectrum estimation with 7 DPSS windows
    the following metrics will be computed: Debiased WPLI Square
    computing cross-spectral density for epoch 1
Processing epoch : 2 / 72 (at most)
Extracting time courses for 4 labels (mode: mean_flip)
    computing cross-spectral density for epoch 2
Processing epoch : 3 / 72 (at most)
Extracting time courses for 4 labels (mode: mean_flip)
    computing cross-spectral density for epoch 3
Processing epoch : 4 / 72 (at most)
Extracting time courses for 4 labels (mode: mean_flip)
    computing cross-spectral density for epoch 4
Processing epoch : 5 / 72 (at most)
Extracting time courses for 4 labels (mode: mean_flip)
    computing cross-spectral density for epoch 5
Processing epoch : 6 / 72 (at most)
Extracting time courses for 4 labels (mode: mean_flip)
    computing cross-spectral density for epoch 6
Processing epoch : 7 / 72 (at most)
Extracting time courses for 4 labels (mode: mean_flip)
    computing cross-spectral density for epoch 7
Processing epoch : 8 / 72 (at most)
Extracting time courses for 4 labels (mode: mean_flip)
    computing cross-spectral density for epoch 8
Processing epoch : 9 / 72 (at most)
Extracting time courses for 4 labels (mode: mean_flip)
    computing cross-spectral density for epoch 9
Processing epoch : 10 / 72 (at most)
Extracting time courses for 4 labels (mode: mean_flip)
    computing cross-spectral density for epoch 10
    Rejecting  epoch based on EOG : ['EOG 061']
Processing epoch : 11 / 72 (at most)
Extracting time courses for 4 labels (mode: mean_flip)
    computing cross-spectral density for epoch 11
Processing epoch : 12 / 72 (at most)
Extracting time courses for 4 labels (mode: mean_flip)
    computing cross-spectral density for epoch 12
Processing epoch : 13 / 72 (at most)
Extracting time courses for 4 labels (mode: mean_flip)
    computing cross-spectral density for epoch 13
    Rejecting  epoch based on EOG : ['EOG 061']
    Rejecting  epoch based on EOG : ['EOG 061']
Processing epoch : 14 / 72 (at most)
Extracting time courses for 4 labels (mode: mean_flip)
    computing cross-spectral density for epoch 14
Processing epoch : 15 / 72 (at most)
Extracting time courses for 4 labels (mode: mean_flip)
    computing cross-spectral density for epoch 15
Processing epoch : 16 / 72 (at most)
Extracting time courses for 4 labels (mode: mean_flip)
    computing cross-spectral density for epoch 16
Processing epoch : 17 / 72 (at most)
Extracting time courses for 4 labels (mode: mean_flip)
    computing cross-spectral density for epoch 17
    Rejecting  epoch based on EOG : ['EOG 061']
Processing epoch : 18 / 72 (at most)
Extracting time courses for 4 labels (mode: mean_flip)
    computing cross-spectral density for epoch 18
Processing epoch : 19 / 72 (at most)
Extracting time courses for 4 labels (mode: mean_flip)
    computing cross-spectral density for epoch 19
Processing epoch : 20 / 72 (at most)
Extracting time courses for 4 labels (mode: mean_flip)
    computing cross-spectral density for epoch 20
    Rejecting  epoch based on EOG : ['EOG 061']
Processing epoch : 21 / 72 (at most)
Extracting time courses for 4 labels (mode: mean_flip)
    computing cross-spectral density for epoch 21
Processing epoch : 22 / 72 (at most)
Extracting time courses for 4 labels (mode: mean_flip)
    computing cross-spectral density for epoch 22
Processing epoch : 23 / 72 (at most)
Extracting time courses for 4 labels (mode: mean_flip)
    computing cross-spectral density for epoch 23
    Rejecting  epoch based on MAG : ['MEG 1711']
Processing epoch : 24 / 72 (at most)
Extracting time courses for 4 labels (mode: mean_flip)
    computing cross-spectral density for epoch 24
Processing epoch : 25 / 72 (at most)
Extracting time courses for 4 labels (mode: mean_flip)
    computing cross-spectral density for epoch 25
Processing epoch : 26 / 72 (at most)
Extracting time courses for 4 labels (mode: mean_flip)
    computing cross-spectral density for epoch 26
Processing epoch : 27 / 72 (at most)
Extracting time courses for 4 labels (mode: mean_flip)
    computing cross-spectral density for epoch 27
Processing epoch : 28 / 72 (at most)
Extracting time courses for 4 labels (mode: mean_flip)
    computing cross-spectral density for epoch 28
    Rejecting  epoch based on EOG : ['EOG 061']
Processing epoch : 29 / 72 (at most)
Extracting time courses for 4 labels (mode: mean_flip)
    computing cross-spectral density for epoch 29
Processing epoch : 30 / 72 (at most)
Extracting time courses for 4 labels (mode: mean_flip)
    computing cross-spectral density for epoch 30
Processing epoch : 31 / 72 (at most)
Extracting time courses for 4 labels (mode: mean_flip)
    computing cross-spectral density for epoch 31
    Rejecting  epoch based on EOG : ['EOG 061']
Processing epoch : 32 / 72 (at most)
Extracting time courses for 4 labels (mode: mean_flip)
    computing cross-spectral density for epoch 32
Processing epoch : 33 / 72 (at most)
Extracting time courses for 4 labels (mode: mean_flip)
    computing cross-spectral density for epoch 33
    Rejecting  epoch based on EOG : ['EOG 061']
Processing epoch : 34 / 72 (at most)
Extracting time courses for 4 labels (mode: mean_flip)
    computing cross-spectral density for epoch 34
Processing epoch : 35 / 72 (at most)
Extracting time courses for 4 labels (mode: mean_flip)
    computing cross-spectral density for epoch 35
    Rejecting  epoch based on EOG : ['EOG 061']
    Rejecting  epoch based on EOG : ['EOG 061']
Processing epoch : 36 / 72 (at most)
Extracting time courses for 4 labels (mode: mean_flip)
    computing cross-spectral density for epoch 36
    Rejecting  epoch based on EOG : ['EOG 061']
    Rejecting  epoch based on EOG : ['EOG 061']
Processing epoch : 37 / 72 (at most)
Extracting time courses for 4 labels (mode: mean_flip)
    computing cross-spectral density for epoch 37
Processing epoch : 38 / 72 (at most)
Extracting time courses for 4 labels (mode: mean_flip)
    computing cross-spectral density for epoch 38
Processing epoch : 39 / 72 (at most)
Extracting time courses for 4 labels (mode: mean_flip)
    computing cross-spectral density for epoch 39
Processing epoch : 40 / 72 (at most)
Extracting time courses for 4 labels (mode: mean_flip)
    computing cross-spectral density for epoch 40
Processing epoch : 41 / 72 (at most)
Extracting time courses for 4 labels (mode: mean_flip)
    computing cross-spectral density for epoch 41
Processing epoch : 42 / 72 (at most)
Extracting time courses for 4 labels (mode: mean_flip)
    computing cross-spectral density for epoch 42
Processing epoch : 43 / 72 (at most)
Extracting time courses for 4 labels (mode: mean_flip)
    computing cross-spectral density for epoch 43
Processing epoch : 44 / 72 (at most)
Extracting time courses for 4 labels (mode: mean_flip)
    computing cross-spectral density for epoch 44
    Rejecting  epoch based on EOG : ['EOG 061']
Processing epoch : 45 / 72 (at most)
Extracting time courses for 4 labels (mode: mean_flip)
    computing cross-spectral density for epoch 45
    Rejecting  epoch based on EOG : ['EOG 061']
Processing epoch : 46 / 72 (at most)
Extracting time courses for 4 labels (mode: mean_flip)
    computing cross-spectral density for epoch 46
Processing epoch : 47 / 72 (at most)
Extracting time courses for 4 labels (mode: mean_flip)
    computing cross-spectral density for epoch 47
Processing epoch : 48 / 72 (at most)
Extracting time courses for 4 labels (mode: mean_flip)
    computing cross-spectral density for epoch 48
    Rejecting  epoch based on EOG : ['EOG 061']
    Rejecting  epoch based on EOG : ['EOG 061']
Processing epoch : 49 / 72 (at most)
Extracting time courses for 4 labels (mode: mean_flip)
    computing cross-spectral density for epoch 49
Processing epoch : 50 / 72 (at most)
Extracting time courses for 4 labels (mode: mean_flip)
    computing cross-spectral density for epoch 50
Processing epoch : 51 / 72 (at most)
Extracting time courses for 4 labels (mode: mean_flip)
    computing cross-spectral density for epoch 51
Processing epoch : 52 / 72 (at most)
Extracting time courses for 4 labels (mode: mean_flip)
    computing cross-spectral density for epoch 52
Processing epoch : 53 / 72 (at most)
Extracting time courses for 4 labels (mode: mean_flip)
    computing cross-spectral density for epoch 53
Processing epoch : 54 / 72 (at most)
Extracting time courses for 4 labels (mode: mean_flip)
    computing cross-spectral density for epoch 54
Processing epoch : 55 / 72 (at most)
Extracting time courses for 4 labels (mode: mean_flip)
    computing cross-spectral density for epoch 55
[done]
    assembling connectivity matrix
[Connectivity computation done]

# Authors: Alexandre Gramfort <alexandre.gramfort@inria.fr>
#
# License: BSD (3-clause)

import matplotlib.pyplot as plt
import mne
from mne.datasets import sample
from mne.minimum_norm import apply_inverse_epochs, read_inverse_operator

from mne_connectivity import spectral_connectivity_epochs

print(__doc__)

data_path = sample.data_path()
subjects_dir = data_path / "subjects"
fname_inv = data_path / "MEG/sample/sample_audvis-meg-oct-6-meg-inv.fif"
fname_raw = data_path / "MEG/sample/sample_audvis_filt-0-40_raw.fif"
fname_event = data_path / "MEG/sample/sample_audvis_filt-0-40_raw-eve.fif"

# Load data
inverse_operator = read_inverse_operator(fname_inv)
raw = mne.io.read_raw_fif(fname_raw)
events = mne.read_events(fname_event)

# Add a bad channel
raw.info["bads"] += ["MEG 2443"]

# Pick MEG channels
picks = mne.pick_types(
    raw.info, meg=True, eeg=False, stim=False, eog=True, exclude="bads"
)

# Define epochs for left-auditory condition
event_id, tmin, tmax = 1, -0.2, 0.5
epochs = mne.Epochs(
    raw,
    events,
    event_id,
    tmin,
    tmax,
    picks=picks,
    baseline=(None, 0),
    reject=dict(mag=4e-12, grad=4000e-13, eog=150e-6),
)

# Compute inverse solution and for each epoch. By using "return_generator=True"
# stcs will be a generator object instead of a list.
snr = 1.0  # use lower SNR for single epochs
lambda2 = 1.0 / snr**2
method = "dSPM"  # use dSPM method (could also be MNE or sLORETA)
stcs = apply_inverse_epochs(
    epochs, inverse_operator, lambda2, method, pick_ori="normal", return_generator=True
)

# Read some labels
names = ["Aud-lh", "Aud-rh", "Vis-lh", "Vis-rh"]
labels = [
    mne.read_label(data_path / f"MEG/sample/labels/{name}.label") for name in names
]

# Average the source estimates within each label using sign-flips to reduce
# signal cancellations, also here we return a generator
src = inverse_operator["src"]
label_ts = mne.extract_label_time_course(
    stcs, labels, src, mode="mean_flip", return_generator=True
)

fmin, fmax = 7.5, 40.0
sfreq = raw.info["sfreq"]  # the sampling frequency

con = spectral_connectivity_epochs(
    label_ts,
    method="wpli2_debiased",
    mode="multitaper",
    sfreq=sfreq,
    fmin=fmin,
    fmax=fmax,
    mt_adaptive=True,
    n_jobs=1,
)
freqs = con.freqs

n_rows, n_cols = con.get_data(output="dense").shape[:2]
fig, axes = plt.subplots(n_rows, n_cols, sharex=True, sharey=True)
for i in range(n_rows):
    for j in range(i + 1):
        if i == j:
            axes[i, j].set_axis_off()
            continue

        axes[i, j].plot(freqs, con.get_data(output="dense")[i, j, :])
        axes[j, i].plot(freqs, con.get_data(output="dense")[i, j, :])

        if j == 0:
            axes[i, j].set_ylabel(names[i])
            axes[0, i].set_title(names[i])
        if i == (n_rows - 1):
            axes[i, j].set_xlabel(names[j])
        axes[i, j].set(xlim=[fmin, fmax], ylim=[-0.2, 1])
        axes[j, i].set(xlim=[fmin, fmax], ylim=[-0.2, 1])

        # Show band limits
        for f in [8, 12, 18, 35]:
            axes[i, j].axvline(f, color="k")
            axes[j, i].axvline(f, color="k")
plt.tight_layout()
plt.show()

Total running time of the script: (0 minutes 2.657 seconds)

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